rabbit anti hipk3 polyclonal antibody  (Proteintech)

Journal: Scientific reports

Article Title: Overexpression of microRNA-205-5p promotes cholangiocarcinoma growth by reducing expression of homeodomain-interacting protein kinase 3.

doi: 10.1038/s41598-023-49694-x

Figure Lengend Snippet: Figure 4. Target prediction of miR-205-5p identifies HIPK3 as one of the potential targets of miR-205-5p. (a) The miRDB database predicted 736 potential target genes. For the TargetScan and miRWalk databases, the corresponding numbers were 590 and 4636, respectively. In total, 140 potential target genes were identified in common by all three databases. (b) Analysis using existing data from the GEPIA database reveals that the relative expression level of HIPK3 in CCA tissues (n = 36) is higher than in corresponding non-tumorous tissues (n = 9). (c) Kaplan–Meier overall survival curves according to the levels of HIPK3 expression. (d) Expression levels of five predicted target genes (CDK14, CREB1, HIPK3, KMT2A, and PLCB1) in the miR- 205-5p inhibitor-transfected KKU-213B cell line. (e) Relative expression levels of HIPK3 in three different batches of KKU-213B cells transfected with miR-205-5p inhibitor. Experiments were performed in triplicate on each batch and expressed as the mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001).

Article Snippet: The sections were then incubated with rabbit anti-HIPK3 polyclonal antibody (1:400 dilution; cat. No. 25107-1-AP, Proteintech, USA) diluted in 5% BSA solution at 4 °C overnight.

Techniques: Expressing, Transfection

Journal: Scientific reports

Article Title: Overexpression of microRNA-205-5p promotes cholangiocarcinoma growth by reducing expression of homeodomain-interacting protein kinase 3.

doi: 10.1038/s41598-023-49694-x

Figure Lengend Snippet: Figure 5. HIPK3 might be direct target of miR-205-5p. (a) Relative basal expression of HIPK3 in CCA cells (KKU-055, KKU-100, and KKU-213b) and immortalized cholangiocyte cell line (MMNK1) was measured by RT-qPCR. (b) Relative expression of HIPK3 in the KKU-100 CCA cell line was measured at 48 h after transfection with a HIPK3 pcDNA. (c) Relative proliferation rates of KKU-100 after transfected with HIPK3 pcDNA were measured using MTT assay at 24, 48 and 72 h after transfection. (d, e) The relative cell migration rates of KKU-100 cells transfected with HIPK3 pcDNA compared with mock-transfected controls at 12, 24, 48, and 72 h were measured using wound-healing assay. (f, g) KKU-213B cell line was transfected with miR-205-5p inhibitor and (h, i) KKU-100 cell line was transfected with miR-205-5p mimic and the expression of HIPK3 was measured by Western blot analysis. Experiments were performed in triplicate for each batch and expressed as mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Article Snippet: The sections were then incubated with rabbit anti-HIPK3 polyclonal antibody (1:400 dilution; cat. No. 25107-1-AP, Proteintech, USA) diluted in 5% BSA solution at 4 °C overnight.

Techniques: Expressing, Quantitative RT-PCR, Transfection, MTT Assay, Migration, Wound Healing Assay, Western Blot

Journal: Scientific reports

Article Title: Overexpression of microRNA-205-5p promotes cholangiocarcinoma growth by reducing expression of homeodomain-interacting protein kinase 3.

doi: 10.1038/s41598-023-49694-x

Figure Lengend Snippet: Figure 6. HIPK3 immunohistochemical staining in CCA tissues (magnification, × 40). Immunohistochemistry was used to compare the H-scores of HIPK3 in normal and CCA tissues. (a) One entire tissue microarray slide. (b) The normal bile duct (NBD) expressed HIPK3 significantly more than the CCA cells. The CCA cells stained weakly positive (c) or strongly positive (d) for HIPK3 according to H-scores. (e) HIPK3 H-scores were compared between healthy and malignant tissues using the Mann-Whitney U test. The median H-score cutoff was 137. High expression was higher than the median (n = 44), and low expression was lower than the median (n = 44). (f) The overall survival time of patients with different HIPK3 expression levels (H-score) is shown by Kaplan–Meier curve with number of risk. The experiments were expressed as mean ± SD (****p < 0.0001).

Article Snippet: The sections were then incubated with rabbit anti-HIPK3 polyclonal antibody (1:400 dilution; cat. No. 25107-1-AP, Proteintech, USA) diluted in 5% BSA solution at 4 °C overnight.

Techniques: Immunohistochemical staining, Staining, Immunohistochemistry, Microarray, MANN-WHITNEY, Expressing